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  • Scalable microcarrier-based manufacturing of

    2016-10-20u2002·u2002Until a few years ago, the most commonly used media for the expansion of MSC were composed of basal medium supplemented with 10% fetal bovine serum (FBS) (Chen et al., 2006, Mareschi et al., 2006, Sotiropoulou et al., 2006). However, the disadvantages of FBS such as being ill-defined, the wide batch-to-batch variability and, most importantly ...

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  • Expansion in microcarrier-spinner cultures improves the ...

    2016-6-1u2002·u2002Fifty percent medium change was done every other day. After 7 days of expansion in microcarrier-spinner culture, heMSC were dissociated from the microcarriers with 0.25% trypsin-EDTA for 5–15 min at 37°C before fetal bovine serum was added to stop the enzymatic reaction (all from Gibco).

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    Who We Are Big Idea Ventures is solving the World's Biggest Challenges by supporting the World's Best Entrepreneurs. We're a venture capital fund with startup accelerators in key locations, investing in and accelerating top performers in the new food space. NEW PROTEIN FUND AND ACCELERATOR Our first fund, The New Protein Fund I (size USD$50M), […]

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    Corning Incorporated is one of the world's leading innovators in materials science, with a nearly 170-year track record of life-changing inventions and category-defining products

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    Daniel Grande. Ernesto Molmenti. Horacio Rodriguez Rilo. Mesenchymal stromal cells (MSCs) are multipotent progenitor cells that can be isolated and expanded from various sources. MSCs modulate the ...

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    The Sci-Hub project supports Open Access movement in science. Research should be published in open access, i.e. be free to read. The Open Access is a new and advanced form of scientific communication, which is going to replace outdated subscription models.

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    2017-9-21u2002·u2002Now, the 37-year-old entrepreneur behind Hampton Creek is vowing to beat a handful of food-tech start-ups to market a meat made without an animal. 'We're about …

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  • Jacques' Newsletters Sept. 15-24, 2021 HIGHEST RED

    2021-9-24u2002·u2002'Such health concerns, particularly regarding cancer and birth defects thought to be caused by chemical factories, have been a major factor in a recent wave of protests. Among the latest was the demonstration last month by hundreds of people living in a Beijing suburb against plans to build a factory in their neighbourhood.

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  • Scalable microcarrier-based manufacturing of

    2016-10-20u2002·u2002Until a few years ago, the most commonly used media for the expansion of MSC were composed of basal medium supplemented with 10% fetal bovine serum (FBS) (Chen et al., 2006, Mareschi et al., 2006, Sotiropoulou et al., 2006). However, the disadvantages of FBS such as being ill-defined, the wide batch-to-batch variability and, most importantly ...

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    2021-9-22u2002·u2002eLife is a non-profit organisation inspired by research funders and led by scientists. Our mission is to help scientists accelerate discovery by operating a platform for research communication that encourages and recognises the most responsible behaviours in science. eLife Sciences Publications, Ltd is a limited liability non-profit non-stock corporation incorporated in the State of Delaware ...

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  • Can you make meat without an animal? Hampton Creek is ...

    2017-9-21u2002·u2002Now, the 37-year-old entrepreneur behind Hampton Creek is vowing to beat a handful of food-tech start-ups to market a meat made without an animal. 'We're about …

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    Sydney Office. Sigma-Aldrich Pty Ltd, An affiliate of Merck KGaA, Darmstadt, Germany. ABN: 66 051 627 831. PO Box 125. North Ryde BC NSW 1670. Tel: 1800 …

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  • No-kill, lab-grown meat to go on sale for first time ...

    2020-12-1u2002·u2002No-kill, lab-grown meat to go on sale for first time. This article is more than 9 months old. Singapore's approval of chicken cells grown in bioreactors is seen as landmark moment across ...

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    Shop online for Electronics, Computers, Clothing, Shoes, Toys, Books, DVDs, Sporting Goods, Beauty & more.

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  • Jacques' Newsletters Sept. 15-24, 2021 HIGHEST RED

    2021-9-24u2002·u2002'Such health concerns, particularly regarding cancer and birth defects thought to be caused by chemical factories, have been a major factor in a recent wave of protests. Among the latest was the demonstration last month by hundreds of people living in a Beijing suburb against plans to build a factory in their neighbourhood.

    Get Price
  • Scalable microcarrier-based manufacturing of

    2016-10-20u2002·u2002Until a few years ago, the most commonly used media for the expansion of MSC were composed of basal medium supplemented with 10% fetal bovine serum (FBS) (Chen et al., 2006, Mareschi et al., 2006, Sotiropoulou et al., 2006). However, the disadvantages of FBS such as being ill-defined, the wide batch-to-batch variability and, most importantly ...

    Get Price
  • Expansion in microcarrier-spinner cultures improves the ...

    2016-6-1u2002·u2002Fifty percent medium change was done every other day. After 7 days of expansion in microcarrier-spinner culture, heMSC were dissociated from the microcarriers with 0.25% trypsin-EDTA for 5–15 min at 37°C before fetal bovine serum was added to stop the enzymatic reaction (all from Gibco).

    Get Price
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    Thermo Fisher Scientific - US. The impact of Hurricane Ida has caused hazardous conditions across Louisiana. Expect service delays in those areas. See details ›. Gibco media, now with. cell culture plastics to match. Learn more.

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    STEMCELL Technologies develops cell culture media, cell separation systems, instruments and other reagents for use in life sciences research.

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    Dissertations & Theses from 2019. Krishnan, Ankita (2019) Understanding Autism Spectrum Disorder Through a Cultural Lens: Perspectives, Stigma, and Cultural Values among Asians . Suzuki, Takakuni (2019) Quantifying the Relations among Neurophysiological Responses, Dimensional Psychopathology, and Personality Traits . Dissertations & Theses from 2018. Abdel-Salam, Ahmed Nabil (2018) …

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    Contact Us. Online Order Support. Customer Service. Technical Support. Instrument Service. Dealer or Distributor. Website Assistance. Self Service. Register for an account.

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  • Can you make meat without an animal? Hampton Creek is ...

    2017-9-21u2002·u2002Now, the 37-year-old entrepreneur behind Hampton Creek is vowing to beat a handful of food-tech start-ups to market a meat made without an animal. 'We're about …

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  • Jacques' Newsletters Sept. 15-24, 2021 HIGHEST RED

    2021-9-24u2002·u2002'Such health concerns, particularly regarding cancer and birth defects thought to be caused by chemical factories, have been a major factor in a recent wave of protests. Among the latest was the demonstration last month by hundreds of people living in a Beijing suburb against plans to build a factory in their neighbourhood.

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  • Frontiers

    2020-7-15u2002·u2002A freshly isolated skin sample taken from a 59 year old healthy male was dissected and placed in 0.1% gelatin coated 6-well plates containing 1 mL of DMEM High-Glucose Medium (EMD Millipore) with 10% fetal bovine serum (EMD Millipore, Burlington, MA) with 1% GlutaMAX (Life technologies Corporation, Grand Island, NY) at 37°C with 5.0% CO 2. The ...

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  • Scalable microcarrier-based manufacturing of mesenchymal ...

    2016-10-20u2002·u2002Until a few years ago, the most commonly used media for the expansion of MSC were composed of basal medium supplemented with 10% fetal bovine serum (FBS) (Chen et al., 2006, Mareschi et al., 2006, Sotiropoulou et al., 2006). However, the disadvantages of FBS such as being ill-defined, the wide batch-to-batch variability and, most importantly ...

    Get Price
  • Small molecule–driven direct conversion of human ...

    2016-8-1u2002·u2002The abilities of human pluripotent stem cells (hPSCs) to proliferate without phenotypic alteration and to differentiate into tissue-specific progeny make them a promising cell source for regenerative medicine and development of physiologically relevant in vitro platforms. Despite this potential, efficient conversion of hPSCs into tissue-specific cells still remains a challenge.

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  • Regionally-Specified Second Trimester Fetal Neural Stem ...

    2014-9-2u2002·u2002Neurospheres were dissociated as above and cultured in differentiation medium (DMEM-F12 with 1% N2 supplement, 1% fetal bovine serum and 1X antibiotic/antimycotic on coverslips coated with poly-L-ornithine for one week before washing with PBS and fixed with 1∶1 methanol acetone for 5 min at −20°C for analysis.

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  • Pentraxin 3 secreted by human adipose‐derived stem cells ...

    2021-6-21u2002·u2002Blocking was performed with 5% bovine serum albumin (BSA) for 30 minutes for the reduction of nonspecific binding, followed by treatment with (150 µL/well) the primary antibody (1:100 nestin and SOX9, Abcam) diluted in PBS containing 1% BSA and incubation for 2 hours at room temperature, and washing three times with PBS.

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  • Passage and reversal effects on gene expression of bovine ...

    2007-9-13u2002·u2002The knee meniscus contains a mixed population of cells that exhibit fibroblastic as well as chondrocytic characteristics. Tissue engineering studies and future therapies for the meniscus require a large population of cells that are seeded on scaffolds. To achieve this, monolayer expansion is often used as a technique to increase cell number. However, the phenotype of these …

    Get Price
  • No-kill, lab-grown meat to go on sale for first time ...

    2020-12-1u2002·u2002No-kill, lab-grown meat to go on sale for first time. This article is more than 9 months old. Singapore's approval of chicken cells grown in bioreactors is seen as landmark moment across ...

    Get Price
  • Perturbing chondroitin sulfate proteoglycan signaling ...

    2018-3-20u2002·u2002Briefly, postnatal (P1–P3) rat pup cortices were mechanically dissociated and the cells passed first through a 70 μm and then a 45 μm cell strainer. The cell solution was then plated into 75 cm 2 flasks in DMEM/F12 (1:1) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin/neomycin (PSN). Mixed glia cultures were ...

    Get Price
  • Man v food: is lab-grown meat really going to solve our ...

    2021-7-29u2002·u2002The sales pitch for the technology is classic Silicon Valley: unseat an obsolete technology – in this case, animals – and do well by doing good. Intensive animal agriculture, which produces nuggets and most of the other meat that Americans consume, keeps the price of meat artificially low by operating at huge economies of scale, and ...

    Get Price
  • Serum IgG-induced microglial activation enhances neuronal ...

    2020-6-16u2002·u2002Opsoclonus-myoclonus syndrome (OMS) is a rare neurological disease. Some children with OMS also have neuroblastoma (NB). We and others have previously documented that serum IgG from children with OMS and NB induces neuronal cytolysis and activates several signaling pathways. However, the mechanisms underlying OMS remain unclear. Here, we investigated …

    Get Price
  • Frontiers

    2020-7-15u2002·u2002A freshly isolated skin sample taken from a 59 year old healthy male was dissected and placed in 0.1% gelatin coated 6-well plates containing 1 mL of DMEM High-Glucose Medium (EMD Millipore) with 10% fetal bovine serum (EMD Millipore, Burlington, MA) with 1% GlutaMAX (Life technologies Corporation, Grand Island, NY) at 37°C with 5.0% CO 2. The ...

    Get Price
  • Scalable microcarrier-based manufacturing of

    2016-10-20u2002·u2002Until a few years ago, the most commonly used media for the expansion of MSC were composed of basal medium supplemented with 10% fetal bovine serum (FBS) (Chen et al., 2006, Mareschi et al., 2006, Sotiropoulou et al., 2006). However, the disadvantages of FBS such as being ill-defined, the wide batch-to-batch variability and, most importantly ...

    Get Price
  • Small molecule–driven direct conversion of human ...

    2016-8-1u2002·u2002The abilities of human pluripotent stem cells (hPSCs) to proliferate without phenotypic alteration and to differentiate into tissue-specific progeny make them a promising cell source for regenerative medicine and development of physiologically relevant in vitro platforms. Despite this potential, efficient conversion of hPSCs into tissue-specific cells still remains a challenge.

    Get Price
  • Regionally-Specified Second Trimester Fetal Neural Stem ...

    2014-9-2u2002·u2002Neurospheres were dissociated as above and cultured in differentiation medium (DMEM-F12 with 1% N2 supplement, 1% fetal bovine serum and 1X antibiotic/antimycotic on coverslips coated with poly-L-ornithine for one week before washing with PBS and fixed with 1∶1 methanol acetone for 5 min at −20°C for analysis.

    Get Price
  • Pentraxin 3 secreted by human adipose‐derived stem cells ...

    2021-6-21u2002·u2002Blocking was performed with 5% bovine serum albumin (BSA) for 30 minutes for the reduction of nonspecific binding, followed by treatment with (150 µL/well) the primary antibody (1:100 nestin and SOX9, Abcam) diluted in PBS containing 1% BSA and incubation for 2 hours at room temperature, and washing three times with PBS.

    Get Price
  • Passage and reversal effects on gene expression of bovine ...

    2007-9-13u2002·u2002The knee meniscus contains a mixed population of cells that exhibit fibroblastic as well as chondrocytic characteristics. Tissue engineering studies and future therapies for the meniscus require a large population of cells that are seeded on scaffolds. To achieve this, monolayer expansion is often used as a technique to increase cell number. However, the phenotype of these cells may be ...

    Get Price
  • No-kill, lab-grown meat to go on sale for first time ...

    2020-12-1u2002·u2002No-kill, lab-grown meat to go on sale for first time. This article is more than 9 months old. Singapore's approval of chicken cells grown in bioreactors is seen as landmark moment across ...

    Get Price
  • Perturbing chondroitin sulfate proteoglycan signaling ...

    2018-3-20u2002·u2002Briefly, postnatal (P1–P3) rat pup cortices were mechanically dissociated and the cells passed first through a 70 μm and then a 45 μm cell strainer. The cell solution was then plated into 75 cm 2 flasks in DMEM/F12 (1:1) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin/neomycin (PSN). Mixed glia cultures were ...

    Get Price
  • Man v food: is lab-grown meat really going to solve our ...

    2021-7-29u2002·u2002The sales pitch for the technology is classic Silicon Valley: unseat an obsolete technology – in this case, animals – and do well by doing good. Intensive animal agriculture, which produces nuggets and most of the other meat that Americans consume, keeps the price of meat artificially low by operating at huge economies of scale, and ...

    Get Price
  • Serum IgG-induced microglial activation enhances

    2020-6-16u2002·u2002Opsoclonus-myoclonus syndrome (OMS) is a rare neurological disease. Some children with OMS also have neuroblastoma (NB). We and others have previously documented that serum IgG from children with OMS and NB induces neuronal cytolysis and activates several signaling pathways. However, the mechanisms underlying OMS remain unclear. Here, we investigated whether nitric oxide …

    Get Price
  • Frontiers

    2020-7-15u2002·u2002A freshly isolated skin sample taken from a 59 year old healthy male was dissected and placed in 0.1% gelatin coated 6-well plates containing 1 mL of DMEM High-Glucose Medium (EMD Millipore) with 10% fetal bovine serum (EMD Millipore, Burlington, MA) with 1% GlutaMAX (Life technologies Corporation, Grand Island, NY) at 37°C with 5.0% CO 2. The ...

    Get Price
  • Scalable microcarrier-based manufacturing of

    2016-10-20u2002·u2002Until a few years ago, the most commonly used media for the expansion of MSC were composed of basal medium supplemented with 10% fetal bovine serum (FBS) (Chen et al., 2006, Mareschi et al., 2006, Sotiropoulou et al., 2006). However, the disadvantages of FBS such as being ill-defined, the wide batch-to-batch variability and, most importantly ...

    Get Price
  • Small molecule–driven direct conversion of human ...

    2016-8-1u2002·u2002The abilities of human pluripotent stem cells (hPSCs) to proliferate without phenotypic alteration and to differentiate into tissue-specific progeny make them a promising cell source for regenerative medicine and development of physiologically relevant in vitro platforms. Despite this potential, efficient conversion of hPSCs into tissue-specific cells still remains a challenge.

    Get Price
  • Regionally-Specified Second Trimester Fetal Neural Stem ...

    2014-9-2u2002·u2002Neurospheres were dissociated as above and cultured in differentiation medium (DMEM-F12 with 1% N2 supplement, 1% fetal bovine serum and 1X antibiotic/antimycotic on coverslips coated with poly-L-ornithine for one week before washing with PBS and fixed with 1∶1 methanol acetone for 5 min at −20°C for analysis.

    Get Price
  • Pentraxin 3 secreted by human adipose‐derived stem cells ...

    2021-6-21u2002·u2002Blocking was performed with 5% bovine serum albumin (BSA) for 30 minutes for the reduction of nonspecific binding, followed by treatment with (150 µL/well) the primary antibody (1:100 nestin and SOX9, Abcam) diluted in PBS containing 1% BSA and incubation for 2 hours at room temperature, and washing three times with PBS.

    Get Price
  • Passage and reversal effects on gene expression of bovine ...

    2007-9-13u2002·u2002The knee meniscus contains a mixed population of cells that exhibit fibroblastic as well as chondrocytic characteristics. Tissue engineering studies and future therapies for the meniscus require a large population of cells that are seeded on scaffolds. To achieve this, monolayer expansion is often used as a technique to increase cell number. However, the phenotype of these cells may be ...

    Get Price
  • No-kill, lab-grown meat to go on sale for first time ...

    2020-12-1u2002·u2002No-kill, lab-grown meat to go on sale for first time. This article is more than 9 months old. Singapore's approval of chicken cells grown in bioreactors is seen as landmark moment across ...

    Get Price
  • Perturbing chondroitin sulfate proteoglycan signaling ...

    2018-3-20u2002·u2002Briefly, postnatal (P1–P3) rat pup cortices were mechanically dissociated and the cells passed first through a 70 μm and then a 45 μm cell strainer. The cell solution was then plated into 75 cm 2 flasks in DMEM/F12 (1:1) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin/neomycin (PSN). Mixed glia cultures were ...

    Get Price
  • Man v food: is lab-grown meat really going to solve our ...

    2021-7-29u2002·u2002The sales pitch for the technology is classic Silicon Valley: unseat an obsolete technology – in this case, animals – and do well by doing good. Intensive animal agriculture, which produces nuggets and most of the other meat that Americans consume, keeps the price of meat artificially low by operating at huge economies of scale, and ...

    Get Price
  • Serum IgG-induced microglial activation enhances

    2020-6-16u2002·u2002Opsoclonus-myoclonus syndrome (OMS) is a rare neurological disease. Some children with OMS also have neuroblastoma (NB). We and others have previously documented that serum IgG from children with OMS and NB induces neuronal cytolysis and activates several signaling pathways. However, the mechanisms underlying OMS remain unclear. Here, we investigated whether nitric oxide …

    Get Price