Overview of the Purification of Recombinant Proteins
Sonication is also useful for breaking cells but is best suited for volumes <100 ml. Alternatively, the outer cell wall can be enzymatically digested with lysozyme (200 µg/ml) and the cells broken by detergents, sonication, or both (Kaback, 1971; Burgess and Jendrisak, 1975; UNIT 6.5).
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