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  • (PDF) A Method for Differentiating Fetal Bovine Serum

    A Method for Differentiating Fetal Bovine Serum f rom Newborn Calf Serum. by adding the mean GGT value of 100 % NBCS (81 4.33 IU /L) and FBS (5 IU/L) and multi plying by the dilution factors ...

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  • Optimization of chemically defined cell culture media ...

    2010-6-1u2002·u2002Now, the cultures are kept in Serum Optimem for the entire time of culture, though good replacement for this serum-containing medium is preferred. Other groups have experienced similar difficulties using B27 ( Cressey, 2009 ), and an American group has recently published the recipe for a new supplement, N21, a re-defined and modified ...

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  • Google Scholar

    Google Scholar provides a simple way to broadly search for scholarly literature. Search across a wide variety of disciplines and sources: articles, theses, books, abstracts and court opinions.

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  • BMC, research in progress - BioMed Central

    BMC, research in progress. A pioneer of open access publishing, BMC has an evolving portfolio of high quality peer-reviewed journals including broad interest titles such as BMC Biology and BMC Medicine, specialist journals such as Malaria Journal and Microbiome, and the BMC Series. Expanding beyond biomedicine into the physical sciences ...

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  • Cell Culture Media: A Review - labome.com

    2021-8-26u2002·u2002Presence of serum in the media has many drawbacks and can lead to serious misinterpretations in immunological studies [2, 3].A number of serum-free media have been developed [4, 5].These media are generally specifically formulated to support the culture of a single cell type, such as Knockout Serum Replacement and Knockout DMEM from Thermo Fisher Scientific, and mTESR …

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  • Official Home Page : Bausch + Lomb

    Coupons and Offers Find current money-saving coupons and other offers available for our products! Science Award and Scholarships The Bausch + Lomb Honorary Science Award honors high school science students. A Lifetime of Healthy Vision A lifetime of healthy vision-don't take it for granted.

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  • pcbn inserts in solid cbn and tipped cbn for hard turning ...

    2021-7-20u2002·u2002swift manufacturing ltd offer precision cutting tools of pcbn inserts, we supply with solid cbn inserts, solid corner cbn inserts and tipped cbn inserts, mainly for machining ferrous metals like cast iron and hardened steel from continuous turning to heavy interrupted turning. As cbn material has advantages in heat absorption capacity and resistance to impact and wear, cbn inserts are used for ...

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  • Efficient generation of dopaminergic induced neuronal ...

    2021-7-13u2002·u2002The enzyme TH is a critical but not absolutely specific marker of midbrain dopamine neurons. Peripheral neurons, such as the secondary neurons of the peripheral sympathetic nervous system or neurons within the enteric system, require TH for catecholamine biosynthesis and stain positive for this marker (Eisenhofer et al., 2004).Of note, while Ascl1 plays important roles in the CNS, it is also ...

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  • A convenient protocol for establishing a human cell ...

    2018-7-18u2002·u2002The optic nerve is severed at the nerve head to detach the retina. The retinae are pooled in KCl buffer (0.3M KCl, 10mM HEPES, 0.5mM CaCl2, 1mM MgCl2; pH 7.0) with 48% sucrose (w/v) and agitated vigorously for 2 minutes on a rotation mixer after which the solution is …

    Get Price
  • New Scientist

    Science news and science articles from New Scientist

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  • Human Platelet Lysate Can Replace Fetal Calf Serum as a ...

    Fetal calf serum (FCS) is frequently used as a growth factor and protein source in bone-marrow-derived mesenchymal stromal cell (BMSC) culture media, although it is a xenogenic product presenting multiple disadvantages including but not limited to ethical concerns. A promising alternative for FCS is human platelet lysate (hPL), which is produced out of human platelet concentrates and happens ...

    Get Price
  • Colostrum for the Dairy Calf - Ontario

    2021-2-13u2002·u2002The most important factor in dairy calf health and survival is feeding the newborn calf adequate amounts of high-quality colostrum early in its life. The cost of raising replacement dairy animals increases if calf-rearing results in higher-than-normal mortality or …

    Get Price
  • Assessment of biomarkers for precision dairy calf rearing ...

    2020-8-5u2002·u2002As such, calf serum IgG levels peak in the first 24 hours and subsequently gradually decline to minimum levels at around 4 weeks of age (Logan et al., 1973). Following the reduction in serum IgG derived from passive immunity, IgG serum levels increase again as calves begin to rely on the development of their own adaptive immune system.

    Get Price
  • CytivaHyClone Cosmic Calf Serum (New Zealand

    Cytiva. SH30413.02HI. Heat Inactivated Cosmic Calf Serum, New Zealand origin. 500 mL. This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000. View substitute products ». SH3041302IR.

    Get Price
  • Calf's Choice Total Colostrum HiCal - RJ Matthews

    Calf's Choice Total HiCal contains min 100 gm globulin protein and 20% fat. Directions: Replacement Colostrum - Mix 1-2 bags (700 gms) with 5-10 cups hot water. Supplemental Colostrum - Mix 1/2-1 bag with 2.5-5 cups hot water. Feed as soon as possible after birth by bottle or esophageal feeder.

    Get Price
  • Safety and therapeutic potential of human bone marrow ...

    2021-5-11u2002·u2002PB-MSCs are isolated by Ficoll-Paque density-gradient separation method using the peripheral blood and are cultured in α-MEM supplemented with 10% newborn calf serum (NBCS). MSCs derived from other sources such as the bronchoalveolar lavage (BAL) fluid, atrium tissue from heart, spleen tissue and perirenal fat tissue

    Get Price
  • Knock-Out Serum Replacement and Melatonin Effects on

    2017-8-25u2002·u2002Serum Replacement (KSR), was suggested as a promising approach. However, the efficiency of that model is still not optimal. Hence, we have tried to establish the culture conditions in two laboratories, and to improve the reliability of the culture system to generate mature germ cells. Male mice at three days of age were sacrificed. Testes ...

    Get Price
  • A Comparative Study of Protocols for Mouse Embryonic

    2014-2-5u2002·u2002A Comparative Study of Protocols for Mouse Embryonic Stem Cell Culturing Christoffer Tamm1, Sara Pijuan Galito´1, Cecilia Annere´n1,2* 1Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden, 2GE Healthcare Bio-Sciences AB, Uppsala, Sweden

    Get Price
  • ATP- and UTP-activated P2Y receptors differently

    2019-10-29u2002·u2002ATP- and UTP-activated P2Y receptors differently regulate proliferation of human lung epithelial tumor cells Rainer Scha¨fer,1 Fariba Sedehizade,1 Tobias Welte,2 and Georg Reiser1 1Institut fu¨r Neurobiochemie and 2Klinik fu¨r Kardiologie, Angiologie, und Pneumologie, Medizinische Fakulta¨t, Otto-von-Guericke-Universita¨t, 39120 Magdeburg, Germany

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  • Stem Cell 2011;2(1) - sciencepub.net

    2011-8-7u2002·u2002modified Eagle's medium, 10% fetal calf serum (FCS), penicillin (100 U/ml), and streptomycin (100 µg/ml) (All provided by Invitrogen). After 3 days of incubation, non-adherent cells were collected and washed with -free medium and counted. - Flow cytometric …

    Get Price
  • Human Platelet Lysate Can Replace Fetal Calf Serum as a ...

    Fetal calf serum (FCS) is frequently used as a growth factor and protein source in bone-marrow-derived mesenchymal stromal cell (BMSC) culture media, although it is a xenogenic product presenting multiple disadvantages including but not limited to ethical concerns. A promising alternative for FCS is human platelet lysate (hPL), which is produced out of human platelet concentrates and happens ...

    Get Price
  • Colostrum for the Dairy Calf - Ontario

    2021-2-13u2002·u2002The most important factor in dairy calf health and survival is feeding the newborn calf adequate amounts of high-quality colostrum early in its life. The cost of raising replacement dairy animals increases if calf-rearing results in higher-than-normal mortality or …

    Get Price
  • Assessment of biomarkers for precision dairy calf rearing ...

    2020-8-5u2002·u2002As such, calf serum IgG levels peak in the first 24 hours and subsequently gradually decline to minimum levels at around 4 weeks of age (Logan et al., 1973). Following the reduction in serum IgG derived from passive immunity, IgG serum levels increase again as calves begin to rely on the development of their own adaptive immune system.

    Get Price
  • CytivaHyClone Cosmic Calf Serum (New Zealand

    Cytiva. SH30413.02HI. Heat Inactivated Cosmic Calf Serum, New Zealand origin. 500 mL. This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000. View substitute products ». SH3041302IR.

    Get Price
  • Calf's Choice Total Colostrum HiCal - RJ Matthews

    Calf's Choice Total HiCal contains min 100 gm globulin protein and 20% fat. Directions: Replacement Colostrum - Mix 1-2 bags (700 gms) with 5-10 cups hot water. Supplemental Colostrum - Mix 1/2-1 bag with 2.5-5 cups hot water. Feed as soon as possible after birth by bottle or esophageal feeder.

    Get Price
  • Safety and therapeutic potential of human bone marrow ...

    2021-5-11u2002·u2002PB-MSCs are isolated by Ficoll-Paque density-gradient separation method using the peripheral blood and are cultured in α-MEM supplemented with 10% newborn calf serum (NBCS). MSCs derived from other sources such as the bronchoalveolar lavage (BAL) fluid, atrium tissue from heart, spleen tissue and perirenal fat tissue

    Get Price
  • Knock-Out Serum Replacement and Melatonin Effects on

    2017-8-25u2002·u2002Serum Replacement (KSR), was suggested as a promising approach. However, the efficiency of that model is still not optimal. Hence, we have tried to establish the culture conditions in two laboratories, and to improve the reliability of the culture system to generate mature germ cells. Male mice at three days of age were sacrificed. Testes ...

    Get Price
  • A Comparative Study of Protocols for Mouse Embryonic

    2014-2-5u2002·u2002A Comparative Study of Protocols for Mouse Embryonic Stem Cell Culturing Christoffer Tamm1, Sara Pijuan Galito´1, Cecilia Annere´n1,2* 1Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden, 2GE Healthcare Bio-Sciences AB, Uppsala, Sweden

    Get Price
  • ATP- and UTP-activated P2Y receptors differently

    2019-10-29u2002·u2002ATP- and UTP-activated P2Y receptors differently regulate proliferation of human lung epithelial tumor cells Rainer Scha¨fer,1 Fariba Sedehizade,1 Tobias Welte,2 and Georg Reiser1 1Institut fu¨r Neurobiochemie and 2Klinik fu¨r Kardiologie, Angiologie, und Pneumologie, Medizinische Fakulta¨t, Otto-von-Guericke-Universita¨t, 39120 Magdeburg, Germany

    Get Price
  • Stem Cell 2011;2(1) - sciencepub.net

    2011-8-7u2002·u2002modified Eagle's medium, 10% fetal calf serum (FCS), penicillin (100 U/ml), and streptomycin (100 µg/ml) (All provided by Invitrogen). After 3 days of incubation, non-adherent cells were collected and washed with -free medium and counted. - Flow cytometric …

    Get Price
  • Human Platelet Lysate Can Replace Fetal Calf Serum as a ...

    Fetal calf serum (FCS) is frequently used as a growth factor and protein source in bone-marrow-derived mesenchymal stromal cell (BMSC) culture media, although it is a xenogenic product presenting multiple disadvantages including but not limited to ethical concerns. A promising alternative for FCS is human platelet lysate (hPL), which is produced out of human platelet …

    Get Price
  • Colostrum for the Dairy Calf - Ontario

    2021-2-13u2002·u2002The most important factor in dairy calf health and survival is feeding the newborn calf adequate amounts of high-quality colostrum early in its life. The cost of raising replacement dairy animals increases if calf-rearing results in higher-than-normal mortality or requires medicine to treat preventable diseases.

    Get Price
  • Assessment of biomarkers for precision dairy calf rearing ...

    2020-8-5u2002·u2002As such, calf serum IgG levels peak in the first 24 hours and subsequently gradually decline to minimum levels at around 4 weeks of age (Logan et al., 1973). Following the reduction in serum IgG derived from passive immunity, IgG serum levels increase again as calves begin to rely on the development of their own adaptive immune system.

    Get Price
  • CytivaHyClone Cosmic Calf Serum (New Zealand

    Cytiva. SH30413.02HI. Heat Inactivated Cosmic Calf Serum, New Zealand origin. 500 mL. This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000. View substitute products ». SH3041302IR.

    Get Price
  • Calf's Choice Total Colostrum HiCal - RJ Matthews

    Calf's Choice Total HiCal contains min 100 gm globulin protein and 20% fat. Directions: Replacement Colostrum - Mix 1-2 bags (700 gms) with 5-10 cups hot water. Supplemental Colostrum - Mix 1/2-1 bag with 2.5-5 cups hot water. Feed as soon as possible after birth by bottle or esophageal feeder.

    Get Price
  • Safety and therapeutic potential of human bone marrow ...

    2021-5-11u2002·u2002PB-MSCs are isolated by Ficoll-Paque density-gradient separation method using the peripheral blood and are cultured in α-MEM supplemented with 10% newborn calf serum (NBCS). MSCs derived from other sources such as the bronchoalveolar lavage (BAL) fluid, atrium tissue from heart, spleen tissue and perirenal fat tissue

    Get Price
  • Knock-Out Serum Replacement and Melatonin Effects

    2017-8-25u2002·u2002Serum Replacement (KSR), was suggested as a promising approach. However, the efficiency of that model is still not optimal. Hence, we have tried to establish the culture conditions in two laboratories, and to improve the reliability of the culture system to generate mature germ cells. Male mice at three days of age were sacrificed. Testes ...

    Get Price
  • A Comparative Study of Protocols for Mouse Embryonic

    2014-2-5u2002·u2002A Comparative Study of Protocols for Mouse Embryonic Stem Cell Culturing Christoffer Tamm1, Sara Pijuan Galito´1, Cecilia Annere´n1,2* 1Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden, 2GE …

    Get Price
  • ATP- and UTP-activated P2Y receptors differently

    2019-10-29u2002·u2002ATP- and UTP-activated P2Y receptors differently regulate proliferation of human lung epithelial tumor cells Rainer Scha¨fer,1 Fariba Sedehizade,1 Tobias Welte,2 and Georg Reiser1 1Institut fu¨r Neurobiochemie and 2Klinik fu¨r Kardiologie, Angiologie, und Pneumologie, Medizinische Fakulta¨t, Otto-von-Guericke-Universita¨t, 39120 Magdeburg, Germany

    Get Price
  • Stem Cell 2011;2(1) - sciencepub.net

    2011-8-7u2002·u2002modified Eagle's medium, 10% fetal calf serum (FCS), penicillin (100 U/ml), and streptomycin (100 µg/ml) (All provided by Invitrogen). After 3 days of incubation, non-adherent cells were collected and washed with -free medium and counted. - Flow cytometric analysis for cell surface markers of BM-MSCs and -HSCs

    Get Price
  • Neonatal Scours, Antibiotics, and Dairy Calves ...

    2021-9-10u2002·u2002Learning Outcomes. This submodule aims to introduce neonatal scours and antibiotic use in dairy calves. By the end of the module, you will be able to: 1. describe the management practices that can prevent scours in calves, such as high-quality colostrum management, sanitation, and proper isolation procedures.

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  • Human mesenchymal stem cells - current trends and

    2015-4-28u2002·u2002The primary culture media was supplemented with 10% FBS [25,33], new-born calf serum (NBCS) or fetal calf serum (FCS) (Table 1). Besides the culture media and supplementation, the oxygen concentration also affects the expansion and proliferation of MSCs .

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  • Factors Affecting Serum Total Protein and ... - Longdom

    2019-4-18u2002·u2002Measurement of serum Total Protein (TP) or Immunoglobulin G (IgG) is frequently used within the dairy industry to monitor failure of passive transfer (FPT) in calves. Through such monitoring, modifications to colostrum . handling and feeding techniques can be incorporated into farm practices to improve calf management. Most studies

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  • Products

    2021-9-5u2002·u2002Frequency ranges from .01-250 pps,(with the ability to effect the nerves by stimulating or facilitating), but also using a High Definition frequency generator (HDfg)™ that can produce much higher frequencies (or pulses per second) from 5,000 pps, 8,300 pps to as high as 10,000 pps to inhibit (interrupt) the pain signals.

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  • B3 Commercial collection of fetal bovine serum

    2019-5-2u2002·u2002free of micro-organisms and this gives the fetal calf its commercial value. Fetal bovine serum is a biological product obtained from the blood of fetal calves. Serum is the component of blood left after the red blood cells have been removed (the blood is allowed to clot, the tubes are centrifuged and the serum is pipetted off).

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  • Erythropoietin-Derived Nonerythropoietic Peptide ...

    2014-3-6u2002·u2002Experimental autoimmune neuritis (EAN) is an autoantigen-specific T-cell-mediated disease model for human demyelinating inflammatory disease of the peripheral nervous system. Erythropoietin (EPO) has been known to promote EAN recovery but its haematopoiesis stimulating effects may limit its clinic application. Here we investigated the effects and potential …

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    customerservice-macys.com

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  • A convenient protocol for establishing a human cell ...

    2018-7-18u2002·u2002The optic nerve is severed at the nerve head to detach the retina. The retinae are pooled in KCl buffer (0.3M KCl, 10mM HEPES, 0.5mM CaCl2, 1mM MgCl2; pH 7.0) with 48% sucrose (w/v) and agitated vigorously for 2 minutes on a rotation mixer after which the solution is centrifuged for 5 minutes at 5,000g.

    Get Price
  • Exosomes derived from preadipocytes improve

    serum (obtained by ultracentrifugation of serum at 100,000 x g for 14 h at 4˚C). Isolation of exosomes. In accordance with a previously reported method (17), cell culture supernatants were collected and centrifuged at 300 x g for 10 min, 1,200 x g for 20 min, and 10,000 x g for 30 min at 4˚C. The supernatant from the

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  • Tissue Engineering in Otorhinolaryngology

    The addition of serum, for example, fetal calf serum (FCS), or growth factors is usually necessary for the proliferation of cells. At the same time, however, these mitogenic stimuli lead to dedifferentiation of cells and tissues in vitro (92,115). To achieve differentiation of proliferated cells for tissue engineer-

    Get Price
  • Human Platelet Lysate Can Replace Fetal Calf Serum as a ...

    Fetal calf serum (FCS) is frequently used as a growth factor and protein source in bone-marrow-derived mesenchymal stromal cell (BMSC) culture media, although it is a xenogenic product presenting multiple disadvantages including but not limited to ethical concerns. A promising alternative for FCS is human platelet lysate (hPL), which is produced out of human platelet concentrates and happens ...

    Get Price
  • Colostrum for the Dairy Calf - Ontario

    2021-2-13u2002·u2002The most important factor in dairy calf health and survival is feeding the newborn calf adequate amounts of high-quality colostrum early in its life. The cost of raising replacement dairy animals increases if calf-rearing results in higher-than-normal mortality or …

    Get Price
  • Assessment of biomarkers for precision dairy calf rearing ...

    2020-8-5u2002·u2002As such, calf serum IgG levels peak in the first 24 hours and subsequently gradually decline to minimum levels at around 4 weeks of age (Logan et al., 1973). Following the reduction in serum IgG derived from passive immunity, IgG serum levels increase again as calves begin to rely on the development of their own adaptive immune system.

    Get Price
  • CytivaHyClone Cosmic Calf Serum (New Zealand

    Cytiva. SH30413.02HI. Heat Inactivated Cosmic Calf Serum, New Zealand origin. 500 mL. This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000. View substitute products ». SH3041302IR.

    Get Price
  • Calf's Choice Total Colostrum HiCal - RJ Matthews

    Calf's Choice Total HiCal contains min 100 gm globulin protein and 20% fat. Directions: Replacement Colostrum - Mix 1-2 bags (700 gms) with 5-10 cups hot water. Supplemental Colostrum - Mix 1/2-1 bag with 2.5-5 cups hot water. Feed as soon as possible after birth by bottle or esophageal feeder.

    Get Price
  • Safety and therapeutic potential of human bone marrow ...

    2021-5-11u2002·u2002PB-MSCs are isolated by Ficoll-Paque density-gradient separation method using the peripheral blood and are cultured in α-MEM supplemented with 10% newborn calf serum (NBCS). MSCs derived from other sources such as the bronchoalveolar lavage (BAL) fluid, atrium tissue from heart, spleen tissue and perirenal fat tissue

    Get Price
  • Knock-Out Serum Replacement and Melatonin Effects on

    2017-8-25u2002·u2002Serum Replacement (KSR), was suggested as a promising approach. However, the efficiency of that model is still not optimal. Hence, we have tried to establish the culture conditions in two laboratories, and to improve the reliability of the culture system to generate mature germ cells. Male mice at three days of age were sacrificed. Testes ...

    Get Price
  • A Comparative Study of Protocols for Mouse Embryonic

    2014-2-5u2002·u2002A Comparative Study of Protocols for Mouse Embryonic Stem Cell Culturing Christoffer Tamm1, Sara Pijuan Galito´1, Cecilia Annere´n1,2* 1Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden, 2GE Healthcare Bio-Sciences AB, Uppsala, Sweden

    Get Price
  • ATP- and UTP-activated P2Y receptors differently

    2019-10-29u2002·u2002ATP- and UTP-activated P2Y receptors differently regulate proliferation of human lung epithelial tumor cells Rainer Scha¨fer,1 Fariba Sedehizade,1 Tobias Welte,2 and Georg Reiser1 1Institut fu¨r Neurobiochemie and 2Klinik fu¨r Kardiologie, Angiologie, und Pneumologie, Medizinische Fakulta¨t, Otto-von-Guericke-Universita¨t, 39120 Magdeburg, Germany

    Get Price
  • Stem Cell 2011;2(1) - sciencepub.net

    2011-8-7u2002·u2002modified Eagle's medium, 10% fetal calf serum (FCS), penicillin (100 U/ml), and streptomycin (100 µg/ml) (All provided by Invitrogen). After 3 days of incubation, non-adherent cells were collected and washed with -free medium and counted. - Flow cytometric …

    Get Price