A general protocol for the generation of Nanobodies for ...
Comparisons to other approaches. Here we present a general protocol for the generation, selection and purification of recombinant in vivo matured Nanobodies for structural biology 1-5,7,9,19-30 that takes 3-4 months. Our Nanobody discovery platform has the competitive advantage over other recombinant crystallization chaperones 31-33 that the cloned Nanobody library represents the …
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